Guidelines for Abstract Submission

Learn how to correctly send your Abstract

BBEST_Fundo

General information for abstract submission

GENERAL RULES

  • You will be able to submit your abstract only after registering for the conference.
  • Abstracts can be submitted only electronically. To upload it, click on ABSTRACTS.
 

AUTHORS

  • The author submitting the abstract will appear as the first author. The authorship order can be changed when registering new authors by selecting the “Author Position” field.
  • Click on ADD ANOTHER AUTHOR until you complete the authors of your abstract. If the author is already registered in our database, start typing the name in “Author name,” and the system will bring up options. 
  • The main author is equivalent to the first author;
  • Indicate the presenter;
  • Fill in each author’s Institution, city, state, and country fields.
  • Author’s full name and CPF or e-mail will be requested for internal control.

ABSTRACT SUBMISSION

  • The abstract must be written in English.
  • Insert the title – limit of 255 (two hundred and fifty-five) characters;
  • Select the area of knowledge;
  • Select how the abstract will be presented: Poster or Oral
  • Report the funding agency. If any, specify the funding agency and project number;
  • Insert the abstract text – limit of 2000 (two thousand) characters with space. It should not include tables, graphs, or figures.
  • Include three keywords in the indicated fields;
  • Click save.
  • You will receive an automatic email with your Abstract code, title, and abstract.

ABSTRACT EVALUATION

  • Abstracts will be evaluated considering the following:
    • Scope of the conference;
    • Scientific content (originality, relevance, and quality);
    • Writing quality.
  • The Conference will not accept:
    • Abstracts in Portuguese (or languages other than English);
    • Abstracts that are not explicit in their objectives, results, and conclusions;
    • Lack of clarity, relevance, and originality.
  • Notification of acceptance or refusal will be available in the restricted area in “My Abstracts.”
  1. The submission of abstracts is only possible after registration.
  2. The author/presenter of the paper must be registered for the Congress.
  3. An author may register and present only ONE abstract; however, they may co-author others.
  4. Only abstracts of unpublished papers may be submitted.
  5. Project descriptions, paper intentions and published papers will not be accepted.
  6. The abstract must be written in English.
  7. The submitted abstracts will be analyzed by the Congress Scientific Committee and the result will be made available on the event website by participant’s name or CPF number.
  8. The analysis encompasses the following aspects: relevant, clearly stated objectives; appropriate methodology; clearly presented results; pertinent conclusions. The text quality (grammar, spelling and typing) is the author’s responsibility and is one of the Scientific Committee’s evaluation criteria.
  9. After July 30th, the abstract submission forms for oral presentation and poster presentations will be unavailable.
  10. The abstracts selected for the poster presentation should be displayed at the Poster Session, in English, during the event. The designated area for each poster will be a 1,20 m x 0,90m panel.
  11. The Poster must have a hanging cord and/or adhesive tape to be held in place.

Selection of abstracts for oral or poster presentation

The Abstracts submitted to BBEST & IEA Bioenergy 2024 Conference may be selected for Oral presentations or may be presented as Poster. The Scientific Program Committee will select Abstracts for Oral presentations and the authors will be informed about the form of presentation through the BBEST & IEA Bioenergy 2024 website. The authors must indicate their preferred form of presentation (Oral or Poster) during the registration and abstract submission procedures.

Presentation areas (Abstracts)

A) Biomass: traditional and alternative energy feedstocks, including waste use

B) Biofuel Technologies, including biomass process engineering and biofuels production

C) Biorefineries, Bio-Based Chemicals, Bioproducts and Biomaterials.

D) Biofuel End Use and Engines

E) Sustainability, Environmental, Social, Economic and Public Policy Issues

Abstract model

The number of words (from introduction to conclusion) must be between 200 and 450 in either MS-Word program files (.doc or .docx).

Abstract example

Dual targeting of RNA-binding proteins to mitochondria and chloroplasts

Morgante, CV1,2; Otto, PA3; Silva-Filho, MC1; Gualberto, JM2,3.

 

1 Escola Superior de Agricultura Luiz de Queiroz, USP, São Paulo, SP
2 Centre Nacional de La Recherche Scientifique (CNRS), Estrasburgo, França
3 Instituto de Biociências, USP, São Paulo, SP

 

A massive transfer of mitochondria and chloroplast DNA to the cell nucleus occurred during the evolution of these organelles. As a consequence, most of their proteins are encoded by nuclear genes and have specific N-terminal targeting sequences. Although cell compartmentalization enables distinct roles to organelles, they may have overlapping functions, and a given protein may be required to more than one compartment. Dual targeting appears as a strategy to deal with this requirement. Approximately 30 Arabidopsis thaliana proteins are known to be dual targeted, and most of them are involved in transcription, translation, and oxidative metabolism. Herein we describe the dual targeting of three RNA-binding proteins of A. thaliana to mitochondria and chloroplasts. These proteins are characterized by a conserved RNA recognition motif (RRM) and a C-terminal glycine-rich sequence. Proteins containing a RRM motif are involved in post-transcriptional processes and regulation of gene expression. Using a GFP approach and bombardment of Nicotiana benthamiana mesophyl cells, we demonstrated the dual targeting of the RBP1a (At4g13850), RBP1b (At3g23830), and RPS19 (At5g47320) proteins. Their N-terminal amino acid sequences are relatively conserved, suggesting that they originated by gene duplication after acquisition of the targeting sequence (TS). When the first 39 amino acids of RBP1b were deleted, the protein was localized only to the cytosol, indicating that the signal for RBP1b targeting is located in its N-terminal region. In order to identify residues important for chloroplast and/or mitochondria targeting, we introduced point mutations and deletions into conserved residues of RBP1b TS, and evaluated their effect in the relative mitochondria/chloroplast targeting, using a novel GFP quantitative approach supported by statistical analyses. Mutations and deletions of serine residues along the TS had no effect on the dual targeting of RBP1b, although chloroplast-targeting sequences are normally rich in serine residues. However, mutations of arginine and lysine at the N-terminal region reduced targeting to mitochondria, pointing to the involvement of positive residues in the protein targeting to this organelle. Deletion of the first part of the TS (amino acids 2 to 17) abolished RBP1b targeting to mitochondria, and the protein was localized to the chloroplasts and cytosol. When the second part of the TS (amino acids 18-30) was deleted, RBP1b was detected, but with lower intensity, in chloroplasts, mitochondria and cytosol. Therefore, the signal for RBP1b targeting to chloroplasts is located along the TS, and targeting to mitochondria depends on the first part of the TS. While the first part of RBP1b TS has the information to promote the protein dual targeting, the second part appears to be involved with the efficiency of the process.

 

Keywords: Arabidopsis, GFP, point mutations

 

Financial Support: CNPq and the Centre National de La Recherche Scientifique (CNRS)- France

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